Description
Principle of the assay: The MyBioSource human E-Cadherin ELISA kit is based on standard sandwich enzyme-linked immunesorbent assay technology. Human E-Cadherin specific-specific polyclonal antibodies are precoated onto 96- well plates. The human specific detection polyclonal antibodies are biotinylated. The unknowns and biotinylated detection antibodies are added to the wells subsequently and then followed by washing with prepared wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with prepared wash buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the human E-Cadherin amount of unknown captured in plate